By Doyen T. Nguyen
Movement cytometry immunophenotyping of hematopoietic issues is a posh and significant workout that calls for an outstanding knowing of telephone lineages, developmental pathways, and physiological alterations, in addition to large event in hematopathology. the method contains numerous interrelated phases, from the preliminary scientific choice concerning which hematologic c- dition is acceptable for FCM assay, to the ultimate step of analysis wherein the FCM info is correlated with different proper scientific and laboratory details. the particular FCM trying out includes 3 significant steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring info at the circulate cytometer) and post-analytical (data research and interpretation). The literature, together with the newest FCM textbooks, presents abundant details at the te- nical rules of FCM reminiscent of instrumentation, reagents and laboratory equipment, in addition to qc and caliber coverage. equally, correlations of morphologic findings and p- notypic profiles were good coated in lots of guides. by contrast, less cognizance has been given to the opposite both very important features of FCM immunophenotyping, specifically info research. The latter is an important step during which a phenotypic profile is proven. To bridge this hole within the literature, the point of interest of this booklet is extra on FCM information research than laboratory equipment and technical info. For the reader to familiarize yourself with our info research process, an outline of our method of the pre-analytical and analytical steps is usually provided, with an emphasis at the pre-analytical facets, that have been hardly touched upon within the literature.
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